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Assay Detail

CHEMBL4338928

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse derived homozygous Tg0N4R P301S mutant tau-induced human ON4R tau aggregation harboring P301S mutant transfected in HEK293 cells preincubated for 2 hrs followed by compound addition and measured after 3 hrs by YFP-based fluorescence assay
33
Total Activities
29
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of 4-Piperazine Isoquinoline Derivatives as Potent and Brain-Permeable Tau Prion Inhibitors with CDK8 Activity.
Grandjean JM, Jiu AY, West JW, Aoyagi A, Droege DG, Elepano M, Hirasawa M, Hirouchi M, Murakami R, Lee J, Sasaki K, Hirano S, Ohyama T, Tang BC, Vaz RJ, Inoue M, Olson SH, Prusiner SB, Conrad J, Paras NA.
ACS Med Chem Lett (2020) 11 : 127 -132
PubMed 32071678 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 29 6.72 8.30
fEC50 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4455382 2 8.30
CHEMBL3828637 1 8.05
CHEMBL4584784 2 7.82
CHEMBL4468770 1 7.66
CHEMBL4464358 1 7.46
CHEMBL4473725 1 7.01
CHEMBL4461214 1 6.92
CHEMBL4476483 1 6.92
CHEMBL4456001 2 6.92
CHEMBL4444098 1 6.77
CHEMBL4447689 2 6.55
CHEMBL4516717 1 6.48
CHEMBL4469418 1 6.41
CHEMBL4442184 1 6.40
CHEMBL4457953 1 6.28
CHEMBL4585943 1 6.23
CHEMBL4522858 1 6.17
CHEMBL4593541 1 6.04
CHEMBL4524428 1 5.96
CHEMBL4540965 1 5.85
CHEMBL4529149 1 5.00
CHEMBL4573130 1 -
CHEMBL4559139 1 -
CHEMBL4462872 1 -
CHEMBL4459909 1 -
CHEMBL4557204 1 -
CHEMBL4584145 1 -
CHEMBL4453515 1 -
CHEMBL4444596 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4455382 EC50 = 5.0 nM 8.30
CHEMBL3828637 EC50 = 9.0 nM 8.05
CHEMBL4584784 EC50 = 15.0 nM 7.82
CHEMBL4468770 EC50 = 22.0 nM 7.66
CHEMBL4464358 EC50 = 35.0 nM 7.46
CHEMBL4473725 EC50 = 98.0 nM 7.01
CHEMBL4461214 EC50 = 120.0 nM 6.92
CHEMBL4456001 EC50 = 120.0 nM 6.92
CHEMBL4476483 EC50 = 120.0 nM 6.92
CHEMBL4444098 EC50 = 170.0 nM 6.77
CHEMBL4447689 EC50 = 280.0 nM 6.55
CHEMBL4516717 EC50 = 330.0 nM 6.48
CHEMBL4469418 EC50 = 390.0 nM 6.41
CHEMBL4442184 EC50 = 400.0 nM 6.40
CHEMBL4457953 EC50 = 520.0 nM 6.28
CHEMBL4585943 EC50 = 590.0 nM 6.23
CHEMBL4522858 EC50 = 680.0 nM 6.17
CHEMBL4593541 EC50 = 910.0 nM 6.04
CHEMBL4524428 EC50 = 1100.0 nM 5.96
CHEMBL4540965 EC50 = 1420.0 nM 5.85
CHEMBL4529149 EC50 = 10000.0 nM 5.00
CHEMBL4573130 EC50 > 10000.0 nM -
CHEMBL4559139 EC50 > 10000.0 nM -
CHEMBL4462872 EC50 > 10000.0 nM -
CHEMBL4459909 EC50 > 10000.0 nM -
CHEMBL4456001 fEC50 = 30.0 nM -
CHEMBL4447689 fEC50 = 210.0 nM -
CHEMBL4557204 EC50 > 10000.0 nM -
CHEMBL4584784 fEC50 = 5.0 nM -
CHEMBL4584145 EC50 > 10000.0 nM -
CHEMBL4453515 EC50 > 10000.0 nM -
CHEMBL4455382 fEC50 = 2.0 nM -
CHEMBL4444596 EC50 > 10000.0 nM -