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Assay Detail

CHEMBL4339144

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human NEP expressed in insect cells preincubated for 1 hr using Cys(PT14)-Arg-Arg-Leu-Trp-OH as substrate and measured after 1 hr by fluorescence based assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neprilysin (CHEMBL1944)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Guided Design of Substituted Biphenyl Butanoic Acid Derivatives as Neprilysin Inhibitors.
Kawanami T, Karki RG, Cody E, Liu Q, Liang G, Ksander GM, Rigel DF, Schiering N, Gong Y, Coppola GM, Iwaki Y, Sun R, Neubert A, Fan L, Ingles S, D'Arcy A, Villard F, Ramage P, Jeng AY, Leung-Chu J, Liu J, Beil M, Fu F, Chen W, Cumin F, Wiesmann C, Mogi M.
ACS Med Chem Lett (2020) 11 : 188 -194
PubMed 32071687 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 7.44 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3703484 1 9.52
CHEMBL3676144 1 9.42
CHEMBL4443138 1 9.10
CHEMBL3676150 1 8.82
CHEMBL4557208 1 8.80
CHEMBL4442804 1 8.70
CHEMBL3676141 1 8.35
CHEMBL4518426 1 8.27
CHEMBL417007 LBQ-657 -1.0 1 8.22
CHEMBL3676140 1 8.17
CHEMBL4466321 1 8.16
CHEMBL4474653 1 8.10
CHEMBL4439270 1 7.70
CHEMBL4592467 1 7.67
CHEMBL4434901 1 7.62
CHEMBL3676155 1 7.60
CHEMBL4568947 1 7.58
CHEMBL4475874 1 7.36
CHEMBL4459630 1 7.27
CHEMBL4518025 1 7.22
CHEMBL4547205 1 6.96
CHEMBL4443565 1 6.67
CHEMBL4448802 1 6.56
CHEMBL4564942 1 6.38
CHEMBL46081 1 6.26
CHEMBL4471166 1 6.10
CHEMBL4559586 1 5.86
CHEMBL4465277 1 5.82
CHEMBL4458213 1 5.71
CHEMBL4548017 1 5.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3703484 IC50 = 0.3 nM 9.52
CHEMBL3676144 IC50 = 0.38 nM 9.42
CHEMBL4443138 IC50 = 0.8 nM 9.10
CHEMBL3676150 IC50 = 1.5 nM 8.82
CHEMBL4557208 IC50 = 1.6 nM 8.80
CHEMBL4442804 IC50 = 2.0 nM 8.70
CHEMBL3676141 IC50 = 4.5 nM 8.35
CHEMBL4518426 IC50 = 5.4 nM 8.27
CHEMBL417007 LBQ-657 IC50 = 6.0 nM 8.22
CHEMBL3676140 IC50 = 6.7 nM 8.17
CHEMBL4466321 IC50 = 6.9 nM 8.16
CHEMBL4474653 IC50 = 8.0 nM 8.10
CHEMBL4439270 IC50 = 20.0 nM 7.70
CHEMBL4592467 IC50 = 21.3 nM 7.67
CHEMBL4434901 IC50 = 24.0 nM 7.62
CHEMBL3676155 IC50 = 25.0 nM 7.60
CHEMBL4568947 IC50 = 26.0 nM 7.58
CHEMBL4475874 IC50 = 44.0 nM 7.36
CHEMBL4459630 IC50 = 54.0 nM 7.27
CHEMBL4518025 IC50 = 60.0 nM 7.22
CHEMBL4547205 IC50 = 111.0 nM 6.96
CHEMBL4443565 IC50 = 212.0 nM 6.67
CHEMBL4448802 IC50 = 275.0 nM 6.56
CHEMBL4564942 IC50 = 413.0 nM 6.38
CHEMBL46081 IC50 = 550.0 nM 6.26
CHEMBL4471166 IC50 = 792.0 nM 6.10
CHEMBL4559586 IC50 = 1370.0 nM 5.86
CHEMBL4465277 IC50 = 1500.0 nM 5.82
CHEMBL4458213 IC50 = 1950.0 nM 5.71
CHEMBL4548017 IC50 = 4182.0 nM 5.38
CHEMBL4550194 IC50 = 4862.0 nM 5.31