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Assay Detail

CHEMBL4339582

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal fluorescein-labeled human APC-R3 (1477 to 1519 residues) binding to wild-type C-terminal His6-tagged beta-catenin (138 to 781 residues) expressed in Escherichia coli BL21 DE3 measured after 2.5 hrs by fluorescence polarization assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Catenin beta-1 (CHEMBL5866)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of Peptidomimetics as Selective Inhibitors for the β-Catenin/T-Cell Factor Protein-Protein Interaction.
Wang Z, Zhang M, Wang J, Ji H.
J Med Chem (2019) 62 : 3617 -3635
PubMed 30856332 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 4.06 4.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4451478 1 4.06
CHEMBL4548532 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4451478 Ki = 88000.0 nM 4.06
CHEMBL4548532 Ki = 173000.0 nM -