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Assay Detail

CHEMBL4340709

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human PHD2 using 2OG as substrate and Fe2 as co-factor assessed as hydroxylation incubated for 15 mins in presence of L-ascorbate by LC-MS analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Egl nine homolog 1 (CHEMBL5697)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibition of a viral prolyl hydroxylase.
Langley GW, Abboud MI, Lohans CT, Schofield CJ.
Bioorg Med Chem (2019) 27 : 2405 -2412
PubMed 30737136 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.45 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3646117 1 8.52
CHEMBL3646118 MOLIDUSTAT 2.0 1 8.15
CHEMBL2338329 ROXADUSTAT 4.0 1 7.57
CHEMBL3646221 VADADUSTAT 4.0 1 7.54
CHEMBL4163812 1 7.17
CHEMBL316034 1 5.16
CHEMBL90852 N-OXALYLGLYCINE 1 5.10
CHEMBL1230640 1 4.84
CHEMBL3785164 1 4.02
CHEMBL4540977 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3646117 IC50 = 3.0 nM 8.52
CHEMBL3646118 MOLIDUSTAT IC50 = 7.0 nM 8.15
CHEMBL2338329 ROXADUSTAT IC50 = 27.0 nM 7.57
CHEMBL3646221 VADADUSTAT IC50 = 29.0 nM 7.54
CHEMBL4163812 IC50 = 67.0 nM 7.17
CHEMBL316034 IC50 = 7000.0 nM 5.16
CHEMBL90852 N-OXALYLGLYCINE IC50 = 8000.0 nM 5.10
CHEMBL1230640 IC50 = 14300.0 nM 4.84
CHEMBL3785164 IC50 = 96000.0 nM 4.02
CHEMBL4540977 IC50 > 100000.0 nM -