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Assay Detail

CHEMBL4342533

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human cathepsin B expressed in baculovirus expression system using Z-Arg-Arg-AMC as substrate incubated for 20 mins by fluorescence based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cathepsin B (CHEMBL4072)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cathepsin B: Active site mapping with peptidic substrates and inhibitors.
Schmitz J, Gilberg E, Löser R, Bajorath J, Bartz U, Gütschow M.
Bioorg Med Chem (2019) 27 : 1 -15
PubMed 30473362 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.79 8.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL336228 1 8.74
CHEMBL4582255 1 7.92
CHEMBL342145 1 6.30
CHEMBL344174 1 4.21
CHEMBL138499 1 -
CHEMBL134083 1 -
CHEMBL337090 1 -
CHEMBL141793 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL336228 IC50 = 1.8 nM 8.74
CHEMBL4582255 IC50 = 11.9 nM 7.92
CHEMBL342145 IC50 = 496.0 nM 6.30
CHEMBL344174 IC50 = 62000.0 nM 4.21
CHEMBL138499 IC50 < 10.0 nM -
CHEMBL134083 IC50 < 10.0 nM -
CHEMBL337090 IC50 < 10.0 nM -
CHEMBL141793 IC50 < 10.0 nM -