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Assay Detail

CHEMBL4343403

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BACE in HEK293 cells harboring APP Swedish mutant assessed as reduction in amyloid beta 40 level measured after overnight incubation by sandwich ELISA
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 1 (CHEMBL4822)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of AM-6494: A Potent and Orally Efficacious β-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) Inhibitor with in Vivo Selectivity over BACE2.
Pettus LH, Bourbeau MP, Bradley J, Bartberger MD, Chen K, Hickman D, Johnson M, Liu Q, Manning JR, Nanez A, Siegmund AC, Wen PH, Whittington DA, Allen JR, Wood S.
J Med Chem (2020) 63 : 2263 -2281
PubMed 31589043 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 8.48 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4472708 1 9.52
CHEMBL4577527 1 9.22
CHEMBL4437161 1 9.22
CHEMBL4473740 1 9.15
CHEMBL4468455 1 9.05
CHEMBL4459222 1 8.92
CHEMBL4560579 1 8.92
CHEMBL4475533 1 8.89
CHEMBL4575752 1 8.70
CHEMBL4456863 1 8.68
CHEMBL4456778 1 8.66
CHEMBL4539909 1 8.62
CHEMBL4579155 1 8.47
CHEMBL4593414 1 8.38
CHEMBL4457316 1 8.10
CHEMBL4575413 1 8.08
CHEMBL4460278 1 7.92
CHEMBL4452318 1 7.77
CHEMBL4471141 1 7.35
CHEMBL4461994 1 7.28
CHEMBL4544525 1 7.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4472708 IC50 = 0.3 nM 9.52
CHEMBL4577527 IC50 = 0.6 nM 9.22
CHEMBL4437161 IC50 = 0.6 nM 9.22
CHEMBL4473740 IC50 = 0.7 nM 9.15
CHEMBL4468455 IC50 = 0.9 nM 9.05
CHEMBL4459222 IC50 = 1.2 nM 8.92
CHEMBL4560579 IC50 = 1.2 nM 8.92
CHEMBL4475533 IC50 = 1.3 nM 8.89
CHEMBL4575752 IC50 = 2.0 nM 8.70
CHEMBL4456863 IC50 = 2.1 nM 8.68
CHEMBL4456778 IC50 = 2.2 nM 8.66
CHEMBL4539909 IC50 = 2.4 nM 8.62
CHEMBL4579155 IC50 = 3.4 nM 8.47
CHEMBL4593414 IC50 = 4.2 nM 8.38
CHEMBL4457316 IC50 = 8.0 nM 8.10
CHEMBL4575413 IC50 = 8.4 nM 8.08
CHEMBL4460278 IC50 = 12.0 nM 7.92
CHEMBL4452318 IC50 = 17.0 nM 7.77
CHEMBL4471141 IC50 = 45.0 nM 7.35
CHEMBL4461994 IC50 = 52.0 nM 7.28
CHEMBL4544525 IC50 = 66.0 nM 7.18