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Assay Detail

CHEMBL4343698

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to full length Trypanosoma cruzi Tulahuen C4 C-terminal His-tagged CYP51 expressed in Escherichia coli HMS174 (DE3) assessed as induction of shift in Soret band by spectrophotometry
3
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma cruzi
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Sterol 14-alpha demethylase (CHEMBL1075110)
Type SINGLE PROTEIN
Organism Trypanosoma cruzi (strain CL Brener)

Publication

A new chemotype with promise against Trypanosoma cruzi.
Wang X, Cal M, Kaiser M, Buckner FS, Lepesheva GI, Sanford AG, Wallick AI, Davis PH, Vennerstrom JL.
Bioorg Med Chem Lett (2020) 30 : 126778 -126778
PubMed 31706668 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 2 6.82 7.75
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1397 POSACONAZOLE 4.0 1 7.75
CHEMBL3105168 1 5.88
CHEMBL4448813 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1397 POSACONAZOLE Kd = 18.0 nM 7.75
CHEMBL3105168 Kd = 1320.0 nM 5.88
CHEMBL4448813 Activity - -