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Assay Detail

CHEMBL4344315

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to immobilized biotin-labeled and N-terminal His6-tagged human Mcl-1 expressed in Escherichia coli Rosetta2 DE3 by biolayer interferometry
4
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery and Characterization of 2,5-Substituted Benzoic Acid Dual Inhibitors of the Anti-apoptotic Mcl-1 and Bfl-1 Proteins.
Kump KJ, Miao L, Mady ASA, Ansari NH, Shrestha UK, Yang Y, Pal M, Liao C, Perdih A, Abulwerdi FA, Chinnaswamy K, Meagher JL, Carlson JM, Khanna M, Stuckey JA, Nikolovska-Coleska Z.
J Med Chem (2020) 63 : 2489 -2510
PubMed 31971799 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Activity 2 - -
Kd 2 6.24 6.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4522193 2 6.31
CHEMBL4576854 2 6.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4522193 Kd = 490.0 nM 6.31
CHEMBL4576854 Kd = 670.0 nM 6.17
CHEMBL4522193 Activity - -
CHEMBL4576854 Activity - -