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Assay Detail

CHEMBL4344578

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Binding
Binding affinity to His-tagged PPARalpha LBD (unknown origin) expressed in Escherichia coli Rosetta (DE3) at 25 degree C by isothermal titration calorimetry
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor alpha (CHEMBL239)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Evolution of a 4-Benzyloxy-benzylamino Chemotype to Provide Efficacious, Potent, and Isoform Selective PPARα Agonists as Leads for Retinal Disorders.
Dou X, Nath D, Shin H, Nurmemmedov E, Bourne PC, Ma JX, Duerfeldt AS.
J Med Chem (2020) 63 : 2854 -2876
PubMed 32096640 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 4 5.92 6.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4463341 1 6.85
CHEMBL219586 GW590735 2.0 1 6.77
CHEMBL4564731 1 5.27
CHEMBL4237449 1 4.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4463341 Kd = 140.0 nM 6.85
CHEMBL219586 GW590735 Kd = 170.0 nM 6.77
CHEMBL4564731 Kd = 5320.0 nM 5.27
CHEMBL4237449 Kd = 16900.0 nM 4.77