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Assay Detail

CHEMBL4344595

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine GUS pre-incubated for 5 to 30 mins before 4-methylumbelliferone beta-D-glucuronide substrate addition at pH 4.5
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-glucuronidase (CHEMBL4082)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Substituent Position of Iminocyclitols Determines the Potency and Selectivity for Gut Microbial Xenobiotic-Reactivating Enzymes.
Dashnyam P, Lin HY, Chen CY, Gao S, Yeh LF, Hsieh WC, Tu Z, Lin CH.
J Med Chem (2020) 63 : 4617 -4627
PubMed 32105467 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 5.73 7.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4589187 1 7.32
CHEMBL1232598 1 6.88
CHEMBL4589322 1 5.31
CHEMBL442895 1 4.67
CHEMBL4451253 1 4.49
CHEMBL4441158 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4589187 Ki = 48.0 nM 7.32
CHEMBL1232598 Ki = 132.0 nM 6.88
CHEMBL4589322 Ki = 4870.0 nM 5.31
CHEMBL442895 Ki = 21470.0 nM 4.67
CHEMBL4451253 Ki = 32230.0 nM 4.49
CHEMBL4441158 Ki = 187300.0 nM -