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Assay Detail

CHEMBL4345338

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at C-terminal RLuc8-fused D2 long receptor (unknown origin) transfected in human HEK293T cells co-expressing N-terminal Venus-tagged beta-arrestin2 assessed as increase in beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRET assay
16
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(2) dopamine receptor (CHEMBL217)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Functional-Selectivity Relationship Studies of Novel Apomorphine Analogs to Develop D1R/D2R Biased Ligands.
Park H, Urs AN, Zimmerman J, Liu C, Wang Q, Urs NM.
ACS Med Chem Lett (2020) 11 : 385 -392
PubMed 32184974 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.02 8.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8809 RACLOPRIDE 2.0 2 8.74
CHEMBL4439029 2 7.55
CHEMBL4467483 2 7.52
CHEMBL4442011 2 7.39
CHEMBL4579687 2 7.35
CHEMBL4564103 2 6.50
CHEMBL4513262 2 5.71
CHEMBL4589737 2 5.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8809 RACLOPRIDE IC50 = 1.82 nM 8.74
CHEMBL8809 RACLOPRIDE IC50 = 1.83 nM 8.74
CHEMBL4439029 IC50 = 28.18 nM 7.55
CHEMBL4439029 IC50 = 28.2 nM 7.55
CHEMBL4467483 IC50 = 30.2 nM 7.52
CHEMBL4467483 IC50 = 30.35 nM 7.52
CHEMBL4442011 IC50 = 40.74 nM 7.39
CHEMBL4442011 IC50 = 40.77 nM 7.39
CHEMBL4579687 IC50 = 44.67 nM 7.35
CHEMBL4579687 IC50 = 45.07 nM 7.35
CHEMBL4564103 IC50 = 316.23 nM 6.50
CHEMBL4564103 IC50 = 337.5 nM 6.47
CHEMBL4513262 IC50 = 1949.84 nM 5.71
CHEMBL4513262 IC50 = 1957.0 nM 5.71
CHEMBL4589737 IC50 = 3630.78 nM 5.44
CHEMBL4589737 IC50 = 3629.0 nM 5.44