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Assay Detail

CHEMBL4345633

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human proteasome pre-incubated for 30 mins before Suc-LLVY-AMC substrate addition and measured after 120 mins by fluorescence based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 2 — Organism
Curated By Autocuration

Target

Proteasome (CHEMBL612443)
Type SUBCELLULAR

Publication

Discovery of Peptide Boronate Derivatives as Histone Deacetylase and Proteasome Dual Inhibitors for Overcoming Bortezomib Resistance of Multiple Myeloma.
Zhou Y, Liu X, Xue J, Liu L, Liang T, Li W, Yang X, Hou X, Fang H.
J Med Chem (2020) 63 : 4701 -4715
PubMed 32267687 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.89 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4456886 1 8.96
CHEMBL4527403 1 8.48
CHEMBL4576202 1 8.25
CHEMBL4463372 1 7.80
CHEMBL325041 BORTEZOMIB 3.0 1 7.71
CHEMBL4547369 1 7.51
CHEMBL4527880 1 7.47
CHEMBL4462775 1 6.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4456886 IC50 = 1.1 nM 8.96
CHEMBL4527403 IC50 = 3.3 nM 8.48
CHEMBL4576202 IC50 = 5.6 nM 8.25
CHEMBL4463372 IC50 = 16.0 nM 7.80
CHEMBL325041 BORTEZOMIB IC50 = 19.4 nM 7.71
CHEMBL4547369 IC50 = 31.0 nM 7.51
CHEMBL4527880 IC50 = 33.8 nM 7.47
CHEMBL4462775 IC50 = 110.0 nM 6.96