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Assay Detail

CHEMBL4348490

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged human ALK cytoplasmic domain (1058 to 1620 residues) expressed in Baculovirus expression system using srctide as substrate incubated for 1 hr by mobility shift assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ALK tyrosine kinase receptor (CHEMBL4247)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel mutant-combating ALK and ROS1 dual inhibitors bearing imidazolidin-2-one moiety with reasonable PK properties.
Lei H, Jiang N, Miao X, Xing L, Guo M, Liu Y, Xu H, Gong P, Zuo D, Zhai X.
Eur J Med Chem (2019) 171 : 297 -309
PubMed 30927566 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 8.41 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4470529 1 8.92
CHEMBL4447747 1 8.68
CHEMBL2403108 CERITINIB 4.0 1 8.64
CHEMBL4573193 1 8.57
CHEMBL4466920 1 8.33
CHEMBL4464571 1 8.28
CHEMBL4519408 1 8.17
CHEMBL4465558 1 8.14
CHEMBL601719 CRIZOTINIB 4.0 1 7.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4470529 IC50 = 1.2 nM 8.92
CHEMBL4447747 IC50 = 2.1 nM 8.68
CHEMBL2403108 CERITINIB IC50 = 2.3 nM 8.64
CHEMBL4573193 IC50 = 2.7 nM 8.57
CHEMBL4466920 IC50 = 4.7 nM 8.33
CHEMBL4464571 IC50 = 5.3 nM 8.28
CHEMBL4519408 IC50 = 6.7 nM 8.17
CHEMBL4465558 IC50 = 7.3 nM 8.14
CHEMBL601719 CRIZOTINIB IC50 = 10.3 nM 7.99