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Assay Detail

CHEMBL4349139

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal His6-tagged ITK (352 to 617 residues) expressed in baculovirus infected Sf21 insect cells using STK substrate by HTRF assay
20
Total Activities
17
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase ITK/TSK (CHEMBL2959)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 7H-pyrrolo[2,3-d]pyrimidine derivatives as selective covalent irreversible inhibitors of interleukin-2-inducible T-cell kinase (Itk).
Tang G, Liu L, Wang X, Pan Z.
Eur J Med Chem (2019) 173 : 167 -183
PubMed 30999237 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 8.18 9.30
Ki 3 7.97 8.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4464404 1 9.30
CHEMBL4457018 1 8.89
CHEMBL4593230 1 8.66
CHEMBL4463563 1 8.64
CHEMBL4440621 1 8.44
CHEMBL4443805 2 8.43
CHEMBL4469900 2 8.43
CHEMBL4520286 1 8.29
CHEMBL4438914 1 8.27
CHEMBL4465231 1 8.27
CHEMBL4458344 2 8.24
CHEMBL4562744 1 8.15
CHEMBL4543893 1 8.11
CHEMBL4562542 1 7.26
CHEMBL4574694 1 7.13
CHEMBL4563157 1 6.43
CHEMBL4549626 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4464404 IC50 = 0.5 nM 9.30
CHEMBL4457018 IC50 = 1.3 nM 8.89
CHEMBL4593230 IC50 = 2.2 nM 8.66
CHEMBL4463563 IC50 = 2.3 nM 8.64
CHEMBL4440621 IC50 = 3.6 nM 8.44
CHEMBL4443805 IC50 = 3.7 nM 8.43
CHEMBL4469900 IC50 = 3.7 nM 8.43
CHEMBL4520286 IC50 = 5.1 nM 8.29
CHEMBL4465231 IC50 = 5.4 nM 8.27
CHEMBL4438914 IC50 = 5.4 nM 8.27
CHEMBL4458344 Ki = 5.8 nM 8.24
CHEMBL4443805 Ki = 6.3 nM 8.20
CHEMBL4562744 IC50 = 7.1 nM 8.15
CHEMBL4543893 IC50 = 7.8 nM 8.11
CHEMBL4469900 Ki = 33.7 nM 7.47
CHEMBL4562542 IC50 = 55.0 nM 7.26
CHEMBL4574694 IC50 = 74.0 nM 7.13
CHEMBL4563157 IC50 = 375.0 nM 6.43
CHEMBL4458344 IC50 < 1.26 nM -
CHEMBL4549626 IC50 < 1.26 nM -