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Assay Detail

CHEMBL4349201

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 60 mins followed by substrate addition and measured after 30 mins by horse-radish peroxidase-coupled amplex red reagent-based fluorescence spectrophotometric method
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Amine oxidase [flavin-containing] B (CHEMBL2039)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of coumarin Mannich base derivatives as multifunctional agents against monoamine oxidase B and neuroinflammation for the treatment of Parkinson's disease.
Tao D, Wang Y, Bao XQ, Yang BB, Gao F, Wang L, Zhang D, Li L.
Eur J Med Chem (2019) 173 : 203 -212
PubMed 31005056 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 4.93 8.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL887 RASAGILINE 4.0 1 8.08
CHEMBL4554567 1 5.44
CHEMBL4537471 1 5.33
CHEMBL4439360 1 4.85
CHEMBL4463853 1 4.74
CHEMBL4571403 1 4.70
CHEMBL4466943 1 4.68
CHEMBL4474002 1 4.44
CHEMBL4525381 1 4.39
CHEMBL4550005 1 4.36
CHEMBL4466838 1 4.15
CHEMBL4543997 1 4.03
CHEMBL4541683 1 -
CHEMBL4475218 1 -
CHEMBL4438112 1 -
CHEMBL4546398 1 -
CHEMBL4463260 1 -
CHEMBL4467621 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL887 RASAGILINE IC50 = 8.4 nM 8.08
CHEMBL4554567 IC50 = 3660.0 nM 5.44
CHEMBL4537471 IC50 = 4620.0 nM 5.33
CHEMBL4439360 IC50 = 14080.0 nM 4.85
CHEMBL4463853 IC50 = 18110.0 nM 4.74
CHEMBL4571403 IC50 = 20120.0 nM 4.70
CHEMBL4466943 IC50 = 21100.0 nM 4.68
CHEMBL4474002 IC50 = 35980.0 nM 4.44
CHEMBL4525381 IC50 = 41110.0 nM 4.39
CHEMBL4550005 IC50 = 43670.0 nM 4.36
CHEMBL4466838 IC50 = 71380.0 nM 4.15
CHEMBL4543997 IC50 = 94020.0 nM 4.03
CHEMBL4541683 IC50 > 200000.0 nM -
CHEMBL4475218 IC50 = 151100.0 nM -
CHEMBL4438112 IC50 > 200000.0 nM -
CHEMBL4546398 IC50 > 200000.0 nM -
CHEMBL4463260 IC50 > 200000.0 nM -
CHEMBL4467621 IC50 = 119300.0 nM -