Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4350262

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Trypanosoma brucei PDEB1 catalytic domain (565 to 918 residues) expressed in Escherichia coli BL21 incubated for 20 mins using cAMP as substrate by PDELight HTS cAMP phosphodiesterase Kit based luminometry
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma brucei
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Class 1 phosphodiesterase PDEB1 (CHEMBL2010636)
Type SINGLE PROTEIN
Organism Trypanosoma brucei

Publication

Alkynamide phthalazinones as a new class of TbrPDEB1 inhibitors.
de Heuvel E, Singh AK, Edink E, van der Meer T, van der Woude M, Sadek P, Krell-Jørgensen MP, van den Bergh T, Veerman J, Caljon G, Kalejaiye TD, Wijtmans M, Maes L, de Koning HP, Jan Sterk G, Siderius M, de Esch IJP, Brown DG, Leurs R.
Bioorg Med Chem (2019) 27 : 3998 -4012
PubMed 31327675 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 28 5.94 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4204999 1 7.00
CHEMBL4210475 1 7.00
CHEMBL4519708 1 6.40
CHEMBL4464777 1 6.30
CHEMBL4465919 1 6.20
CHEMBL4473824 1 6.20
CHEMBL4556148 1 6.10
CHEMBL4520514 1 6.10
CHEMBL4467085 1 6.00
CHEMBL4551476 1 5.90
CHEMBL4441002 1 5.90
CHEMBL4543722 1 5.80
CHEMBL4573706 1 5.70
CHEMBL4587960 1 5.70
CHEMBL4449673 1 5.40
CHEMBL4544409 1 5.20
CHEMBL4573906 1 5.10
CHEMBL4476056 1 5.00
CHEMBL4544133 1 -
CHEMBL4445851 1 -
CHEMBL4544549 1 -
CHEMBL4575728 1 -
CHEMBL4532826 1 -
CHEMBL4541444 1 -
CHEMBL4455736 1 -
CHEMBL4527191 1 -
CHEMBL4454672 1 -
CHEMBL4470700 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4204999 Ki = 100.0 nM 7.00
CHEMBL4210475 Ki = 100.0 nM 7.00
CHEMBL4519708 Ki = 398.11 nM 6.40
CHEMBL4464777 Ki = 501.19 nM 6.30
CHEMBL4465919 Ki = 630.96 nM 6.20
CHEMBL4473824 Ki = 630.96 nM 6.20
CHEMBL4556148 Ki = 794.33 nM 6.10
CHEMBL4520514 Ki = 794.33 nM 6.10
CHEMBL4467085 Ki = 1000.0 nM 6.00
CHEMBL4551476 Ki = 1258.93 nM 5.90
CHEMBL4441002 Ki = 1258.93 nM 5.90
CHEMBL4543722 Ki = 1584.89 nM 5.80
CHEMBL4573706 Ki = 1995.26 nM 5.70
CHEMBL4587960 Ki = 1995.26 nM 5.70
CHEMBL4449673 Ki = 3981.07 nM 5.40
CHEMBL4544409 Ki = 6309.57 nM 5.20
CHEMBL4573906 Ki = 7943.28 nM 5.10
CHEMBL4476056 Ki = 10000.0 nM 5.00
CHEMBL4544133 Ki > 10000.0 nM -
CHEMBL4445851 Ki > 10000.0 nM -
CHEMBL4544549 Ki > 10000.0 nM -
CHEMBL4575728 Ki > 10000.0 nM -
CHEMBL4532826 Ki > 10000.0 nM -
CHEMBL4541444 Ki > 10000.0 nM -
CHEMBL4455736 Ki > 10000.0 nM -
CHEMBL4527191 Ki > 10000.0 nM -
CHEMBL4454672 Ki > 10000.0 nM -
CHEMBL4470700 Ki > 10000.0 nM -