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Assay Detail

CHEMBL4350306

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of recombinant human AMPK expressed in Sf9 cells using SAMS peptide as substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins by fluorescence based assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

AMP-activated protein kinase, AMPK (CHEMBL2096907)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

Towards multi-target antidiabetic agents: Discovery of biphenyl-benzimidazole conjugates as AMPK activators.
Babkov DA, Zhukowskaya ON, Borisov AV, Babkova VA, Sokolova EV, Brigadirova AA, Litvinov RA, Kolodina AA, Morkovnik AS, Sochnev VS, Borodkin GS, Spasov AA.
Bioorg Med Chem Lett (2019) 29 : 2443 -2447
PubMed 31358465 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 9.00 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4167177 1 9.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4167177 EC50 = 1.0 nM 9.00