Assay Detail
Binding
CHEMBL4350313
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of C-terminal His tagged human recombinant full length AMPK alpha1/beta1/gamma1 expressed in baculovirus infected Sf9 cells using SAMS peptide as substrate incubated for 60 mins by ADP-Glo luminescence assay
8
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 (CHEMBL2111345) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Towards multi-target antidiabetic agents: Discovery of biphenyl-benzimidazole conjugates as AMPK activators.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 7 | 6.09 | 6.47 |
| pEC50 | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4466855 | — | — | 2 | 6.47 |
| CHEMBL4537753 | — | — | 2 | 6.36 |
| CHEMBL4303623 | — | — | 1 | 6.26 |
| CHEMBL4469542 | — | — | 2 | 4.60 |
| CHEMBL4537786 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4466855 | — | EC50 | = | 340.0 | nM | 6.47 |
| CHEMBL4466855 | — | EC50 | = | 338.84 | nM | 6.47 |
| CHEMBL4537753 | — | EC50 | = | 440.0 | nM | 6.36 |
| CHEMBL4537753 | — | EC50 | = | 446.68 | nM | 6.35 |
| CHEMBL4303623 | — | EC50 | = | 550.0 | nM | 6.26 |
| CHEMBL4469542 | — | EC50 | = | 25118.86 | nM | 4.60 |
| CHEMBL4469542 | — | EC50 | = | 109000.0 | nM | - |
| CHEMBL4537786 | — | pEC50 | - | — | - |