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Assay Detail

CHEMBL4350356

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse recombinant FLAG-tagged PTGES expressed in baculovirus infected Hi-5 insect cells using prostaglandin H2 as substrate incubated for 20 mins followed by substrate addition and incubated for 60 sec by TR-FRET assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E synthase (CHEMBL2046261)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery and optimization of pyridyl-cycloalkyl-carboxylic acids as inhibitors of microsomal prostaglandin E synthase-1 for the treatment of endometriosis.
Koppitz M, Bräuer N, Ter Laak A, Irlbacher H, Rotgeri A, Coelho AM, Walter D, Steinmeyer A, Zollner TM, Peters M, Nagel J.
Bioorg Med Chem Lett (2019) 29 : 2700 -2705
PubMed 31362919 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.20 7.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4459950 1 7.08
CHEMBL4524932 1 6.72
CHEMBL4435689 1 6.69
CHEMBL4443717 1 6.47
CHEMBL4439662 1 6.23
CHEMBL4526207 1 6.12
CHEMBL4467934 1 6.09
CHEMBL4456039 1 5.51
CHEMBL4537349 1 4.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4459950 IC50 = 84.0 nM 7.08
CHEMBL4524932 IC50 = 191.0 nM 6.72
CHEMBL4435689 IC50 = 203.0 nM 6.69
CHEMBL4443717 IC50 = 339.0 nM 6.47
CHEMBL4439662 IC50 = 584.0 nM 6.23
CHEMBL4526207 IC50 = 765.0 nM 6.12
CHEMBL4467934 IC50 = 818.0 nM 6.09
CHEMBL4456039 IC50 = 3080.0 nM 5.51
CHEMBL4537349 IC50 = 12600.0 nM 4.90