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Assay Detail

CHEMBL4351507

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Displacement of [3H]-flumazenil from human GABAA alpha1beta3gamma2 receptor expressed in HEK293 cell membranes measured after 2 hrs by liquid scintillation counting method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-1/beta-3/gamma-2 (CHEMBL2094121)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Design and Identification of a Novel, Functionally Subtype Selective GABA<sub>A</sub> Positive Allosteric Modulator (PF-06372865).
Owen RM, Blakemore D, Cao L, Flanagan N, Fish R, Gibson KR, Gurrell R, Huh CW, Kammonen J, Mortimer-Cassen E, Nickolls SA, Omoto K, Owen D, Pike A, Pryde DC, Reynolds DS, Roeloffs R, Rose C, Stead C, Takeuchi M, Warmus JS, Watson C.
J Med Chem (2019) 62 : 5773 -5796
PubMed 30964988 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 9.47 9.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3647536 DARIGABAT 2.0 1 9.74
CHEMBL4545044 1 9.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3647536 DARIGABAT Ki = 0.18 nM 9.74
CHEMBL4545044 Ki = 0.64 nM 9.19