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Assay Detail

CHEMBL4352728

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Plasmodium falciparum N-terminal His6-tagged falcipain-2 (35 residues) expressed in Escherichia coli M15 pREP4 using Z-Lue-Arg-AMC as substrate measured after 30 mins by spectrofluorometric method
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium falciparum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Falcipain 2 (CHEMBL5800)
Type SINGLE PROTEIN
Organism Plasmodium falciparum

Publication

Falcipain Inhibitors Based on the Natural Product Gallinamide A Are Potent in Vitro and in Vivo Antimalarials.
Stoye A, Juillard A, Tang AH, Legac J, Gut J, White KL, Charman SA, Rosenthal PJ, Grau GER, Hunt NH, Payne RJ.
J Med Chem (2019) 62 : 5562 -5578
PubMed 31062592 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.81 7.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4434685 1 7.54
CHEMBL4467013 1 7.51
CHEMBL4439637 1 7.48
CHEMBL4442854 1 7.24
CHEMBL4472824 1 7.23
CHEMBL4564002 1 7.22
CHEMBL1458713 1 7.17
CHEMBL4442674 1 7.08
CHEMBL4527228 1 6.81
CHEMBL4516951 1 6.77
CHEMBL4437416 1 6.63
CHEMBL4456644 1 6.57
CHEMBL4555968 1 6.34
CHEMBL4475837 1 6.33
CHEMBL4473513 1 5.51
CHEMBL4594166 1 5.45
CHEMBL269671 ARTEMISININ 3.0 1 -
CHEMBL76 CHLOROQUINE 4.0 1 -
CHEMBL4562998 1 -
CHEMBL4555310 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4434685 IC50 = 29.0 nM 7.54
CHEMBL4467013 IC50 = 31.0 nM 7.51
CHEMBL4439637 IC50 = 33.0 nM 7.48
CHEMBL4442854 IC50 = 57.0 nM 7.24
CHEMBL4472824 IC50 = 59.0 nM 7.23
CHEMBL4564002 IC50 = 60.0 nM 7.22
CHEMBL1458713 IC50 = 68.0 nM 7.17
CHEMBL4442674 IC50 = 84.0 nM 7.08
CHEMBL4527228 IC50 = 156.0 nM 6.81
CHEMBL4516951 IC50 = 169.0 nM 6.77
CHEMBL4437416 IC50 = 235.0 nM 6.63
CHEMBL4456644 IC50 = 270.0 nM 6.57
CHEMBL4555968 IC50 = 455.0 nM 6.34
CHEMBL4475837 IC50 = 464.0 nM 6.33
CHEMBL4473513 IC50 = 3097.0 nM 5.51
CHEMBL4594166 IC50 = 3523.0 nM 5.45
CHEMBL269671 ARTEMISININ IC50 - -
CHEMBL76 CHLOROQUINE IC50 - -
CHEMBL4562998 IC50 > 50000.0 nM -
CHEMBL4555310 IC50 > 50000.0 nM -