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Assay Detail

CHEMBL4352750

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full-length N-terminal Flag epitope-tagged ATR expressed in HEK293T cells using ASELPASQPQPFSAKKK peptide as substrate measured after 24 hrs in presence of [gamma-33P] ATP by microbeta liquid scintillation counting analysis
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase ATR (CHEMBL5024)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational Design of 5-(4-(Isopropylsulfonyl)phenyl)-3-(3-(4-((methylamino)methyl)phenyl)isoxazol-5-yl)pyrazin-2-amine (VX-970, M6620): Optimization of Intra- and Intermolecular Polar Interactions of a New Ataxia Telangiectasia Mutated and Rad3-Related (ATR) Kinase Inhibitor.
Knegtel R, Charrier JD, Durrant S, Davis C, O'Donnell M, Storck P, MacCormick S, Kay D, Pinder J, Virani A, Twin H, Griffiths M, Reaper P, Littlewood P, Young S, Golec J, Pollard J.
J Med Chem (2019) 62 : 5547 -5561
PubMed 31074988 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 8.74 9.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4444602 1 9.85
CHEMBL3989870 BERZOSERTIB 2.0 1 9.78
CHEMBL4533440 1 9.68
CHEMBL4443525 1 9.57
CHEMBL4566104 1 9.28
CHEMBL4448538 1 9.12
CHEMBL4434665 1 9.00
CHEMBL4440048 1 9.00
CHEMBL4467820 1 9.00
CHEMBL4467848 1 8.91
CHEMBL4530813 1 8.70
CHEMBL4541587 1 8.62
CHEMBL1766793 1 8.40
CHEMBL4558884 1 8.40
CHEMBL4445532 1 8.30
CHEMBL3695214 1 8.22
CHEMBL4473876 1 7.68
CHEMBL4593939 1 7.58
CHEMBL2409216 1 7.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4444602 Ki = 0.14 nM 9.85
CHEMBL3989870 BERZOSERTIB Ki = 0.165 nM 9.78
CHEMBL4533440 Ki = 0.21 nM 9.68
CHEMBL4443525 Ki = 0.27 nM 9.57
CHEMBL4566104 Ki = 0.53 nM 9.28
CHEMBL4448538 Ki = 0.75 nM 9.12
CHEMBL4434665 Ki = 1.0 nM 9.00
CHEMBL4440048 Ki = 1.0 nM 9.00
CHEMBL4467820 Ki = 1.0 nM 9.00
CHEMBL4467848 Ki = 1.24 nM 8.91
CHEMBL4530813 Ki = 2.0 nM 8.70
CHEMBL4541587 Ki = 2.4 nM 8.62
CHEMBL1766793 Ki = 4.0 nM 8.40
CHEMBL4558884 Ki = 4.0 nM 8.40
CHEMBL4445532 Ki = 5.0 nM 8.30
CHEMBL3695214 Ki = 6.0 nM 8.22
CHEMBL4473876 Ki = 21.0 nM 7.68
CHEMBL4593939 Ki = 26.0 nM 7.58
CHEMBL2409216 Ki = 90.0 nM 7.05