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Assay Detail

CHEMBL4352755

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ATR in human HCT116 cells assessed as reduction in histone H2AX phosphorylation by Hoechst staining-based immunofluorescence microscopic analysis
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase ATR (CHEMBL5024)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational Design of 5-(4-(Isopropylsulfonyl)phenyl)-3-(3-(4-((methylamino)methyl)phenyl)isoxazol-5-yl)pyrazin-2-amine (VX-970, M6620): Optimization of Intra- and Intermolecular Polar Interactions of a New Ataxia Telangiectasia Mutated and Rad3-Related (ATR) Kinase Inhibitor.
Knegtel R, Charrier JD, Durrant S, Davis C, O'Donnell M, Storck P, MacCormick S, Kay D, Pinder J, Virani A, Twin H, Griffiths M, Reaper P, Littlewood P, Young S, Golec J, Pollard J.
J Med Chem (2019) 62 : 5547 -5561
PubMed 31074988 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.58 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4444602 1 7.89
CHEMBL3989870 BERZOSERTIB 2.0 1 7.72
CHEMBL4533440 1 7.12
CHEMBL4541587 1 6.89
CHEMBL4566104 1 6.89
CHEMBL4448538 1 6.68
CHEMBL4467848 1 6.52
CHEMBL4467820 1 6.39
CHEMBL4530813 1 6.10
CHEMBL1766793 1 5.99
CHEMBL4440048 1 5.96
CHEMBL4434665 1 5.77
CHEMBL4445532 1 5.64
CHEMBL4443525 1 -
CHEMBL2409216 1 -
CHEMBL3695214 1 -
CHEMBL4593939 1 -
CHEMBL4558884 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4444602 IC50 = 13.0 nM 7.89
CHEMBL3989870 BERZOSERTIB IC50 = 19.0 nM 7.72
CHEMBL4533440 IC50 = 76.0 nM 7.12
CHEMBL4541587 IC50 = 130.0 nM 6.89
CHEMBL4566104 IC50 = 130.0 nM 6.89
CHEMBL4448538 IC50 = 210.0 nM 6.68
CHEMBL4467848 IC50 = 300.0 nM 6.52
CHEMBL4467820 IC50 = 410.0 nM 6.39
CHEMBL4530813 IC50 = 800.0 nM 6.10
CHEMBL1766793 IC50 = 1030.0 nM 5.99
CHEMBL4440048 IC50 = 1100.0 nM 5.96
CHEMBL4434665 IC50 = 1700.0 nM 5.77
CHEMBL4445532 IC50 = 2300.0 nM 5.64
CHEMBL4443525 IC50 > 25000.0 nM -
CHEMBL2409216 IC50 > 2500.0 nM -
CHEMBL3695214 IC50 > 2500.0 nM -
CHEMBL4593939 IC50 > 2500.0 nM -
CHEMBL4558884 IC50 > 2500.0 nM -