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Assay Detail

CHEMBL4353545

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant N-terminal His-tagged FAK (393 to 698 residues) expressed in baculovirus infected Sf9 insect cells using Poly (4:1 Glu, Tyr) peptide as substrate incubated for 60 mins by ADP-Glo assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Focal adhesion kinase 1 (CHEMBL2695)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2,4-diarylaminopyrimidine derivatives bearing dithiocarbamate moiety as novel FAK inhibitors with antitumor and anti-angiogenesis activities.
Su Y, Li R, Ning X, Lin Z, Zhao X, Zhou J, Liu J, Jin Y, Yin Y.
Eur J Med Chem (2019) 177 : 32 -46
PubMed 31129452 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 7.54 10.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4554455 1 10.15
CHEMBL4469292 1 8.07
CHEMBL4438250 1 7.49
CHEMBL4458861 1 6.76
CHEMBL4453262 1 6.50
CHEMBL4590434 1 6.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4554455 IC50 = 0.07 nM 10.15
CHEMBL4469292 IC50 = 8.57 nM 8.07
CHEMBL4438250 IC50 = 32.7 nM 7.49
CHEMBL4458861 IC50 = 175.4 nM 6.76
CHEMBL4453262 IC50 = 316.3 nM 6.50
CHEMBL4590434 IC50 = 506.7 nM 6.29