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Assay Detail

CHEMBL4355185

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human KLK14 expressed in Spodoptera frugiperda Sf9 cells using Boc-QAR-MCA as susbtrate measured every 60 secs for 10 mins by fluorescence based assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kallikrein-14 (CHEMBL2641)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Amino Acid Scanning at P5' within the Bowman-Birk Inhibitory Loop Reveals Specificity Trends for Diverse Serine Proteases.
Li CY, de Veer SJ, White AM, Chen X, Harris JM, Swedberg JE, Craik DJ.
J Med Chem (2019) 62 : 3696 -3706
PubMed 30888159 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 7.22 7.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4466351 1 7.75
CHEMBL4456620 1 7.10
CHEMBL4575658 1 6.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4466351 Ki = 18.0 nM 7.75
CHEMBL4456620 Ki = 79.0 nM 7.10
CHEMBL4575658 Ki = 150.0 nM 6.82