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Assay Detail

CHEMBL4355674

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant N-terminal GST-tagged human HDAC6 (1 to 1215 residues) expressed in baculovirus infected sf9 insect cells using p53 (379 to 382 residues) derived fluorogenic peptide RHKKAc as substrate preincubated for 5 to 10 mins followed by substrate addition and further incubated for 2 hrs by fluorescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a New Isoxazole-3-hydroxamate-Based Histone Deacetylase 6 Inhibitor SS-208 with Antitumor Activity in Syngeneic Melanoma Mouse Models.
Shen S, Hadley M, Ustinova K, Pavlicek J, Knox T, Noonepalle S, Tavares MT, Zimprich CA, Zhang G, Robers MB, Bařinka C, Kozikowski AP, Villagra A.
J Med Chem (2019) 62 : 8557 -8577
PubMed 31414801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.29 9.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4126811 1 9.48
CHEMBL99 TRICHOSTATIN 1.0 1 8.70
CHEMBL4536605 1 8.64
CHEMBL2179618 NEXTURASTAT A 1 8.30
CHEMBL4554511 1 8.22
CHEMBL4556111 1 8.00
CHEMBL511749 1 7.86
CHEMBL4483795 1 7.62
CHEMBL4447715 1 7.55
CHEMBL4520424 1 7.51
CHEMBL4469488 1 7.12
CHEMBL4584312 1 6.72
CHEMBL4470946 1 6.70
CHEMBL4551620 1 6.16
CHEMBL4526191 1 5.87
CHEMBL4589770 1 5.70
CHEMBL4473037 1 5.70
CHEMBL4561802 1 5.36

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4126811 IC50 = 0.33 nM 9.48
CHEMBL99 TRICHOSTATIN IC50 = 2.0 nM 8.70
CHEMBL4536605 IC50 = 2.3 nM 8.64
CHEMBL2179618 NEXTURASTAT A IC50 = 5.0 nM 8.30
CHEMBL4554511 IC50 = 6.0 nM 8.22
CHEMBL4556111 IC50 = 10.0 nM 8.00
CHEMBL511749 IC50 = 13.8 nM 7.86
CHEMBL4483795 IC50 = 24.0 nM 7.62
CHEMBL4447715 IC50 = 28.0 nM 7.55
CHEMBL4520424 IC50 = 31.0 nM 7.51
CHEMBL4469488 IC50 = 75.0 nM 7.12
CHEMBL4584312 IC50 = 190.0 nM 6.72
CHEMBL4470946 IC50 = 200.0 nM 6.70
CHEMBL4551620 IC50 = 690.0 nM 6.16
CHEMBL4526191 IC50 = 1350.0 nM 5.87
CHEMBL4589770 IC50 = 1980.0 nM 5.70
CHEMBL4473037 IC50 = 1990.0 nM 5.70
CHEMBL4561802 IC50 = 4360.0 nM 5.36