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Assay Detail

CHEMBL4355728

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full length N-terminal GST-tagged HDAC6 expressed in baculovirus infected Sf9 insect cells assessed as decrease in release of 7-amino-4-methoxycoumarin using fluorogenic HDAC as substrate measured after 30 mins by fluorescence based analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a New Isoxazole-3-hydroxamate-Based Histone Deacetylase 6 Inhibitor SS-208 with Antitumor Activity in Syngeneic Melanoma Mouse Models.
Shen S, Hadley M, Ustinova K, Pavlicek J, Knox T, Noonepalle S, Tavares MT, Zimprich CA, Zhang G, Robers MB, Bařinka C, Kozikowski AP, Villagra A.
J Med Chem (2019) 62 : 8557 -8577
PubMed 31414801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.51 7.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4079541 1 7.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4079541 IC50 = 31.0 nM 7.51