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Assay Detail

CHEMBL4356586

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant C-terminal His6x-tagged human Mcl-1 (171 to 327 residues) interaction with biotinylated human Bim (51 to 76 residues) incubated for 60 mins in presence of 0.05 nM biotin-Bim by HTRF assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery and in Vivo Evaluation of Macrocyclic Mcl-1 Inhibitors Featuring an α-Hydroxy Phenylacetic Acid Pharmacophore or Bioisostere.
Rescourio G, Gonzalez AZ, Jabri S, Belmontes B, Moody G, Whittington D, Huang X, Caenepeel S, Cardozo M, Cheng AC, Chow D, Dou H, Jones A, Kelly RC, Li Y, Lizarzaburu M, Lo MC, Mallari R, Meleza C, Rew Y, Simonovich S, Sun D, Turcotte S, Yan X, Wong SG, Yanez E, Zancanella M, Houze J, Medina JC, Hughes PE, Brown SP.
J Med Chem (2019) 62 : 10258 -10271
PubMed 31736296 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 8.72 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4553660 1 9.70
CHEMBL4535151 1 9.70
CHEMBL4580244 1 9.70
CHEMBL4464768 1 9.52
CHEMBL4539543 1 9.52
CHEMBL4446369 1 9.52
CHEMBL4528051 1 9.52
CHEMBL4446378 TAPOTOCLAX 1.0 1 9.52
CHEMBL4438074 1 9.40
CHEMBL4588330 1 9.30
CHEMBL4437832 1 9.30
CHEMBL4577379 1 9.30
CHEMBL4547370 1 9.22
CHEMBL4542646 1 9.22
CHEMBL4593361 1 9.13
CHEMBL4562159 1 9.00
CHEMBL4452794 1 8.96
CHEMBL4469850 1 8.89
CHEMBL4561691 1 8.77
CHEMBL4452403 1 8.30
CHEMBL4446955 1 8.00
CHEMBL4544042 1 8.00
CHEMBL4460664 1 7.96
CHEMBL4522764 1 7.82
CHEMBL4515030 1 7.57
CHEMBL4452437 1 7.46
CHEMBL4574884 1 7.08
CHEMBL4463461 1 6.82
CHEMBL4543603 1 6.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4553660 IC50 = 0.2 nM 9.70
CHEMBL4535151 IC50 = 0.2 nM 9.70
CHEMBL4580244 IC50 = 0.2 nM 9.70
CHEMBL4464768 IC50 = 0.3 nM 9.52
CHEMBL4539543 IC50 = 0.3 nM 9.52
CHEMBL4446369 IC50 = 0.3 nM 9.52
CHEMBL4528051 IC50 = 0.3 nM 9.52
CHEMBL4446378 TAPOTOCLAX IC50 = 0.3 nM 9.52
CHEMBL4438074 IC50 = 0.4 nM 9.40
CHEMBL4588330 IC50 = 0.5 nM 9.30
CHEMBL4437832 IC50 = 0.5 nM 9.30
CHEMBL4577379 IC50 = 0.5 nM 9.30
CHEMBL4547370 IC50 = 0.6 nM 9.22
CHEMBL4542646 IC50 = 0.6 nM 9.22
CHEMBL4593361 IC50 = 0.74 nM 9.13
CHEMBL4562159 IC50 = 1.0 nM 9.00
CHEMBL4452794 IC50 = 1.1 nM 8.96
CHEMBL4469850 IC50 = 1.3 nM 8.89
CHEMBL4561691 IC50 = 1.7 nM 8.77
CHEMBL4452403 IC50 = 5.0 nM 8.30
CHEMBL4446955 IC50 = 10.0 nM 8.00
CHEMBL4544042 IC50 = 10.0 nM 8.00
CHEMBL4460664 IC50 = 11.0 nM 7.96
CHEMBL4522764 IC50 = 15.0 nM 7.82
CHEMBL4515030 IC50 = 27.0 nM 7.57
CHEMBL4452437 IC50 = 35.0 nM 7.46
CHEMBL4574884 IC50 = 83.0 nM 7.08
CHEMBL4463461 IC50 = 153.0 nM 6.82
CHEMBL4543603 IC50 = 281.0 nM 6.55