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Assay Detail

CHEMBL4357478

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Functional
Antagonist activity at mouse melanocortin receptor 5 expressed in HEK293 cells assessed as inhibition of NDP-MSH-induced cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay based Schild analysis
10
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 5 (CHEMBL4489)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Incorporation of Agouti-Related Protein (AgRP) Human Single Nucleotide Polymorphisms (SNPs) in the AgRP-Derived Macrocyclic Scaffold c[Pro-Arg-Phe-Phe-Asn-Ala-Phe-dPro] Decreases Melanocortin-4 Receptor Antagonist Potency and Results in the Discovery of Melanocortin-5 Receptor Antagonists.
Koerperich ZM, Ericson MD, Freeman KT, Speth RC, Pogozheva ID, Mosberg HI, Haskell-Luevano C.
J Med Chem (2020) 63 : 2194 -2208
PubMed 31845801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 6 6.52 7.30
pA2 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4102048 1 7.30
CHEMBL4566163 1 6.50
CHEMBL3577981 1 6.40
CHEMBL4565367 1 6.30
CHEMBL4538274 1 6.10
CHEMBL4587214 1 -
CHEMBL4466724 1 -
CHEMBL4474215 1 -
CHEMBL4531291 1 -
CHEMBL4541505 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4102048 Kd = 50.12 nM 7.30
CHEMBL4566163 Kd = 316.23 nM 6.50
CHEMBL3577981 Kd = 398.11 nM 6.40
CHEMBL4565367 Kd = 501.19 nM 6.30
CHEMBL4538274 Kd = 794.33 nM 6.10
CHEMBL4587214 pA2 - -
CHEMBL4466724 pA2 - -
CHEMBL4474215 Kd > 10000.0 nM -
CHEMBL4531291 pA2 - -
CHEMBL4541505 pA2 - -