Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4357485

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 4 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 4 (CHEMBL3719)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Incorporation of Agouti-Related Protein (AgRP) Human Single Nucleotide Polymorphisms (SNPs) in the AgRP-Derived Macrocyclic Scaffold c[Pro-Arg-Phe-Phe-Asn-Ala-Phe-dPro] Decreases Melanocortin-4 Receptor Antagonist Potency and Results in the Discovery of Melanocortin-5 Receptor Antagonists.
Koerperich ZM, Ericson MD, Freeman KT, Speth RC, Pogozheva ID, Mosberg HI, Haskell-Luevano C.
J Med Chem (2020) 63 : 2194 -2208
PubMed 31845801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.79 8.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 8.89
CHEMBL4102048 1 8.72
CHEMBL4565367 1 8.09
CHEMBL4538274 1 7.42
CHEMBL3577981 1 7.16
CHEMBL4566163 1 6.87
CHEMBL4531291 1 6.00
CHEMBL4474215 1 5.72
CHEMBL4466724 1 5.70
CHEMBL4587214 1 5.17
CHEMBL4541505 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE IC50 = 1.3 nM 8.89
CHEMBL4102048 IC50 = 1.9 nM 8.72
CHEMBL4565367 IC50 = 8.2 nM 8.09
CHEMBL4538274 IC50 = 38.0 nM 7.42
CHEMBL3577981 IC50 = 70.0 nM 7.16
CHEMBL4566163 IC50 = 134.0 nM 6.87
CHEMBL4531291 IC50 = 1000.0 nM 6.00
CHEMBL4474215 IC50 = 1900.0 nM 5.72
CHEMBL4466724 IC50 = 2000.0 nM 5.70
CHEMBL4587214 IC50 = 6700.0 nM 5.17
CHEMBL4541505 IC50 = 10000.0 nM 5.00