Assay Detail
Binding
CHEMBL4357639
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human SphK2 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]S1P production using varying level of D-erythro-sphingosine as substrate in presence of [gamma33P]-ATP by scintillation proximity assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of a Small Side Cavity in Sphingosine Kinase 2 that Enhances Inhibitor Potency and Selectivity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 6 | 6.72 | 7.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4453931 | — | — | 1 | 7.05 |
| CHEMBL4456325 | — | — | 1 | 6.73 |
| CHEMBL4593983 | — | — | 1 | 6.72 |
| CHEMBL4570134 | — | — | 1 | 6.71 |
| CHEMBL4462152 | — | — | 1 | 6.70 |
| CHEMBL4466524 | — | — | 1 | 6.43 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4453931 | — | Ki | = | 89.0 | nM | 7.05 |
| CHEMBL4456325 | — | Ki | = | 186.0 | nM | 6.73 |
| CHEMBL4593983 | — | Ki | = | 192.0 | nM | 6.72 |
| CHEMBL4570134 | — | Ki | = | 197.0 | nM | 6.71 |
| CHEMBL4462152 | — | Ki | = | 201.0 | nM | 6.70 |
| CHEMBL4466524 | — | Ki | = | 370.0 | nM | 6.43 |