Assay Detail
Binding
CHEMBL4358946
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Competitive inhibition of human N-terminal His6-tagged TEV protease site linked DHPS expressed in Escherichia coli BL21 (DE3) assessed as reduction in radiolabelled amino-butylidene incorporation in eIF5A using 2 uM of [3H]Spermidine trichloride as substrate after 90 to 120 mins in presence of eIF5A and 250 uM NAD+ by Topcount scintillation counting method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of Novel Allosteric Inhibitors of Deoxyhypusine Synthase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 4.99 | 6.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL229457 | — | — | 1 | 6.30 |
| CHEMBL4447948 | — | — | 1 | 4.42 |
| CHEMBL4555677 | — | — | 1 | 4.25 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL229457 | — | IC50 | = | 500.0 | nM | 6.30 |
| CHEMBL4447948 | — | IC50 | = | 38000.0 | nM | 4.42 |
| CHEMBL4555677 | — | IC50 | = | 56000.0 | nM | 4.25 |