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Assay Detail

CHEMBL4359199

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NS5A in HCV genotype 1b infected in HuH-Lcu-Neo cells assessed as reduction in viral replication incubated for 48 hrs by luciferase reporter gene assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 1b
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Nonstructural protein 5A (CHEMBL3307224)
Type SINGLE PROTEIN
Organism Hepatitis C virus

Publication

GSK2818713, a Novel Biphenylene Scaffold-Based Hepatitis C NS5A Replication Complex Inhibitor with Broad Genotype Coverage.
Kazmierski WM, Baskaran S, Walker JT, Miriyala N, Meesala R, Beesu M, Adjabeng G, Grimes RM, Hamatake R, Leivers MR, Crosby R, Xia B, Remlinger K.
J Med Chem (2020) 63 : 4155 -4170
PubMed 32202782 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 10.66 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4579455 1 11.00
CHEMBL4549709 1 10.85
CHEMBL4436027 1 10.82
CHEMBL4440257 1 10.77
CHEMBL4561734 1 10.69
CHEMBL4548808 1 10.66
CHEMBL4472037 1 10.55
CHEMBL4466702 1 10.55
CHEMBL4446161 1 10.09
CHEMBL3360467 1 -
CHEMBL4444885 1 -
CHEMBL4483702 1 -
CHEMBL4465921 1 -
CHEMBL4567354 1 -
CHEMBL4448604 1 -
CHEMBL4533823 1 -
CHEMBL4563742 1 -
CHEMBL4531684 1 -
CHEMBL4476378 1 -
CHEMBL2023898 DACLATASVIR 4.0 1 -
CHEMBL4471986 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4579455 EC50 = 0.01 nM 11.00
CHEMBL4549709 EC50 = 0.014 nM 10.85
CHEMBL4436027 EC50 = 0.0151 nM 10.82
CHEMBL4440257 EC50 = 0.0171 nM 10.77
CHEMBL4561734 EC50 = 0.0203 nM 10.69
CHEMBL4548808 EC50 = 0.0217 nM 10.66
CHEMBL4472037 EC50 = 0.0284 nM 10.55
CHEMBL4466702 EC50 = 0.0284 nM 10.55
CHEMBL4446161 EC50 = 0.0807 nM 10.09
CHEMBL3360467 EC50 = 0.0082 nM -
CHEMBL4444885 EC50 = 0.0044 nM -
CHEMBL4483702 EC50 = 0.0057 nM -
CHEMBL4465921 EC50 = 0.0034 nM -
CHEMBL4567354 EC50 = 0.0056 nM -
CHEMBL4448604 EC50 = 0.0093 nM -
CHEMBL4533823 EC50 = 0.0062 nM -
CHEMBL4563742 EC50 = 0.0066 nM -
CHEMBL4531684 EC50 = 0.0078 nM -
CHEMBL4476378 EC50 = 0.0081 nM -
CHEMBL2023898 DACLATASVIR EC50 = 0.0081 nM -
CHEMBL4471986 EC50 = 0.0072 nM -