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Assay Detail

CHEMBL4359941

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human recombinant full length N-terminal His6-tagged IRAK1 (Argl94 to Ser712 residues) expressed in insect cells using H-KKARFSRFAGSSPSQSSMVAR as substrate incubated for 60 mins by fluorescence polarisation assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Interleukin-1 receptor-associated kinase 1 (CHEMBL3357)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Potent and Selective Pyrazolopyrimidine IRAK4 Inhibitors.
Bryan MC, Drobnick J, Gobbi A, Kolesnikov A, Chen Y, Rajapaksa N, Ndubaku C, Feng J, Chang W, Francis R, Yu C, Choo EF, DeMent K, Ran Y, An L, Emson C, Huang Z, Sujatha-Bhaskar S, Brightbill H, DiPasquale A, Maher J, Wai J, McKenzie BS, Lupardus PJ, Zarrin AA, Kiefer JR.
J Med Chem (2019) 62 : 6223 -6240
PubMed 31082230 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 6.30 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4474636 1 7.62
CHEMBL4464832 1 7.02
CHEMBL4568867 1 6.72
CHEMBL4515682 1 6.47
CHEMBL4453861 1 6.40
CHEMBL4551768 1 6.30
CHEMBL3950656 1 6.29
CHEMBL3952937 1 5.55
CHEMBL4460458 1 5.33
CHEMBL4544284 1 5.27
CHEMBL4569648 1 -
CHEMBL3976860 1 -
CHEMBL4554579 1 -
CHEMBL4460055 1 -
CHEMBL4483477 1 -
CHEMBL4436128 1 -
CHEMBL4567291 1 -
CHEMBL3979848 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4474636 Ki = 24.0 nM 7.62
CHEMBL4464832 Ki = 95.0 nM 7.02
CHEMBL4568867 Ki = 190.0 nM 6.72
CHEMBL4515682 Ki = 340.0 nM 6.47
CHEMBL4453861 Ki = 400.0 nM 6.40
CHEMBL4551768 Ki = 500.0 nM 6.30
CHEMBL3950656 Ki = 510.0 nM 6.29
CHEMBL3952937 Ki = 2800.0 nM 5.55
CHEMBL4460458 Ki = 4700.0 nM 5.33
CHEMBL4544284 Ki = 5400.0 nM 5.27
CHEMBL4569648 Ki - -
CHEMBL3976860 Ki - -
CHEMBL4554579 Ki > 5700.0 nM -
CHEMBL4460055 Ki > 5500.0 nM -
CHEMBL4483477 Ki > 6100.0 nM -
CHEMBL4436128 Ki > 10000.0 nM -
CHEMBL4567291 Ki - -
CHEMBL3979848 Ki - -