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Assay Detail

CHEMBL4361018

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human placental SAH hydrolase expressed in Escherichia coli JM109 using SAH as substrate preincubated for 10 mins followed by SAH addition and measured after 20 mins by DNTB reagent-based spectrophotometric method
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosylhomocysteinase (CHEMBL2664)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Anti-RNA Virus Activity of 6'-Fluorinated-Aristeromycin Analogues.
Yoon JS, Kim G, Jarhad DB, Kim HR, Shin YS, Qu S, Sahu PK, Kim HO, Lee HW, Wang SB, Kong YJ, Chang TS, Ogando NS, Kovacikova K, Snijder EJ, Posthuma CC, van Hemert MJ, Jeong LS.
J Med Chem (2019) 62 : 6346 -6362
PubMed 31244113 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.80 6.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2115462 1 6.43
CHEMBL4538845 1 6.12
CHEMBL4544781 1 5.97
CHEMBL49935 ARISTEROMYCIN 1 5.88
CHEMBL4465968 1 5.36
CHEMBL2115031 1 5.01
CHEMBL4456225 1 -
CHEMBL4538994 1 -
CHEMBL4589829 1 -
CHEMBL4591427 1 -
CHEMBL4464364 1 -
CHEMBL4522602 1 -
CHEMBL4473818 1 -
CHEMBL4476535 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2115462 IC50 = 370.0 nM 6.43
CHEMBL4538845 IC50 = 760.0 nM 6.12
CHEMBL4544781 IC50 = 1060.0 nM 5.97
CHEMBL49935 ARISTEROMYCIN IC50 = 1320.0 nM 5.88
CHEMBL4465968 IC50 = 4390.0 nM 5.36
CHEMBL2115031 IC50 = 9700.0 nM 5.01
CHEMBL4456225 IC50 > 100000.0 nM -
CHEMBL4538994 IC50 > 100000.0 nM -
CHEMBL4589829 IC50 > 100000.0 nM -
CHEMBL4591427 IC50 > 100000.0 nM -
CHEMBL4464364 IC50 > 100000.0 nM -
CHEMBL4522602 IC50 > 100000.0 nM -
CHEMBL4473818 IC50 > 100000.0 nM -
CHEMBL4476535 IC50 > 100000.0 nM -