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Assay Detail

CHEMBL4361683

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]epibatidine from alpha4beta2 nAChR in rat cerebral cortex membrane
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cerebral cortex
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Novel N-aryl nicotinamide derivatives: Taking stock on 3,6-diazabicyclo[3.1.1]heptanes as ligands for neuronal acetylcholine receptors.
Murineddu G, Gotti C, Asproni B, Corona P, Martinello K, Plutino S, Fucile S, Temml V, Moretti M, Viani P, Schuster D, Piras S, Deligia F, Pinna GA.
Eur J Med Chem (2019) 180 : 51 -61
PubMed 31299587 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 9.66 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4460815 1 11.00
CHEMBL4543232 1 10.51
CHEMBL4441630 1 10.38
CHEMBL4565381 1 10.36
CHEMBL4556523 1 10.29
CHEMBL4444866 1 9.97
CHEMBL4581470 1 9.71
CHEMBL4453342 1 9.50
CHEMBL4435435 1 9.44
CHEMBL4447564 1 9.39
CHEMBL4532589 1 9.25
CHEMBL4462607 1 9.08
CHEMBL4530364 1 9.01
CHEMBL4571320 1 8.96
CHEMBL4476500 1 8.89
CHEMBL4472986 1 8.74
CHEMBL298826 (-)-EPIBATIDINE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4460815 Ki = 0.01 nM 11.00
CHEMBL4543232 Ki = 0.031 nM 10.51
CHEMBL4441630 Ki = 0.042 nM 10.38
CHEMBL4565381 Ki = 0.044 nM 10.36
CHEMBL4556523 Ki = 0.051 nM 10.29
CHEMBL4444866 Ki = 0.106 nM 9.97
CHEMBL4581470 Ki = 0.196 nM 9.71
CHEMBL4453342 Ki = 0.315 nM 9.50
CHEMBL4435435 Ki = 0.366 nM 9.44
CHEMBL4447564 Ki = 0.404 nM 9.39
CHEMBL4532589 Ki = 0.56 nM 9.25
CHEMBL4462607 Ki = 0.828 nM 9.08
CHEMBL4530364 Ki = 0.985 nM 9.01
CHEMBL4571320 Ki = 1.1 nM 8.96
CHEMBL4476500 Ki = 1.3 nM 8.89
CHEMBL4472986 Ki = 1.8 nM 8.74
CHEMBL298826 (-)-EPIBATIDINE Ki = 5e-05 nM -