Assay Detail
Binding
CHEMBL4362335
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of TrxR in human SH-SY5Y cells using DTNB as substrate preincubated for 15 mins followed by substrate addition and measured for 20 mins by Ellman's method
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | SH-SY5Y |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Thioredoxin reductase 1, cytoplasmic (CHEMBL1927) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Novel electrophilic amides amenable by the Ugi reaction perturb thioredoxin system via thioredoxin reductase 1 (TrxR1) inhibition: Identification of DVD-445 as a new lead compound for anticancer therapy.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 14 | 4.56 | 5.14 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4459518 | — | — | 1 | 5.14 |
| CHEMBL4589636 | — | — | 1 | 5.07 |
| CHEMBL4577957 | — | — | 1 | 4.80 |
| CHEMBL4562297 | — | — | 1 | 4.76 |
| CHEMBL4544484 | — | — | 1 | 4.53 |
| CHEMBL4465914 | — | — | 1 | 4.51 |
| CHEMBL4452177 | — | — | 1 | 4.50 |
| CHEMBL465843 | PIPLARTINE | — | 1 | 4.34 |
| CHEMBL4451049 | — | — | 1 | 4.29 |
| CHEMBL4515694 | — | — | 1 | 4.12 |
| CHEMBL4460514 | — | — | 1 | 4.09 |
| CHEMBL4443061 | — | — | 1 | - |
| CHEMBL4447931 | — | — | 1 | - |
| CHEMBL4439743 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4459518 | — | IC50 | = | 7260.0 | nM | 5.14 |
| CHEMBL4589636 | — | IC50 | = | 8580.0 | nM | 5.07 |
| CHEMBL4577957 | — | IC50 | = | 16010.0 | nM | 4.80 |
| CHEMBL4562297 | — | IC50 | = | 17440.0 | nM | 4.76 |
| CHEMBL4544484 | — | IC50 | = | 29300.0 | nM | 4.53 |
| CHEMBL4465914 | — | IC50 | = | 30540.0 | nM | 4.51 |
| CHEMBL4452177 | — | IC50 | = | 31840.0 | nM | 4.50 |
| CHEMBL465843 | PIPLARTINE | IC50 | = | 45880.0 | nM | 4.34 |
| CHEMBL4451049 | — | IC50 | = | 51240.0 | nM | 4.29 |
| CHEMBL4515694 | — | IC50 | = | 76620.0 | nM | 4.12 |
| CHEMBL4460514 | — | IC50 | = | 81630.0 | nM | 4.09 |
| CHEMBL4443061 | — | IC50 | > | 200000.0 | nM | - |
| CHEMBL4447931 | — | IC50 | > | 200000.0 | nM | - |
| CHEMBL4439743 | — | IC50 | > | 200000.0 | nM | - |