Assay Detail
Binding
CHEMBL4367522
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as induction of IL2-stimulated cell proliferation by measuring increase in CD3+ cells preincubated for 3 days followed by 11 days incubation post compound washout and subsequent IL2 stimulation for every 3 days measured after 14 days by flow cytometry
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Butyrophilin subfamily 3 member A1 (CHEMBL4105758) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Probing the Ligand-Binding Pocket of BTN3A1.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 11 | 6.63 | 9.29 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL145233 | — | — | 1 | 9.29 |
| CHEMBL4088948 | — | — | 1 | 8.27 |
| CHEMBL4061531 | — | — | 1 | 7.39 |
| CHEMBL4302450 | — | — | 1 | 7.07 |
| CHEMBL4060737 | — | — | 1 | 5.40 |
| CHEMBL4116247 | — | — | 1 | 4.58 |
| CHEMBL356362 | ISOPENTENYLPYROPHOSPHATE | — | 1 | 4.44 |
| CHEMBL343480 | DIMETHYLALLYL DIPHOSPHATE | — | 1 | - |
| CHEMBL4521062 | — | — | 1 | - |
| CHEMBL4531623 | — | — | 1 | - |
| CHEMBL450079 | SODIUM PYROPHOSPHATE | -1.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL145233 | — | EC50 | = | 0.51 | nM | 9.29 |
| CHEMBL4088948 | — | EC50 | = | 5.4 | nM | 8.27 |
| CHEMBL4061531 | — | EC50 | = | 41.0 | nM | 7.39 |
| CHEMBL4302450 | — | EC50 | = | 86.0 | nM | 7.07 |
| CHEMBL4060737 | — | EC50 | = | 4000.0 | nM | 5.40 |
| CHEMBL4116247 | — | EC50 | = | 26000.0 | nM | 4.58 |
| CHEMBL356362 | ISOPENTENYLPYROPHOSPHATE | EC50 | = | 36000.0 | nM | 4.44 |
| CHEMBL343480 | DIMETHYLALLYL DIPHOSPHATE | EC50 | - | — | - | |
| CHEMBL4521062 | — | EC50 | - | — | - | |
| CHEMBL4531623 | — | EC50 | - | — | - | |
| CHEMBL450079 | SODIUM PYROPHOSPHATE | EC50 | - | — | - |