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Assay Detail

CHEMBL4367534

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Binding
Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 B30.2 domain (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins by fluorescence polarization assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Butyrophilin subfamily 3 member A1 (CHEMBL4105758)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Probing the Ligand-Binding Pocket of BTN3A1.
Poe MM, Agabiti SS, Liu C, Li V, Teske KA, Hsiao CC, Wiemer AJ.
J Med Chem (2019) 62 : 6814 -6823
PubMed 31268699 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.87 5.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4302450 1 5.10
CHEMBL145233 1 4.64
CHEMBL343480 DIMETHYLALLYL DIPHOSPHATE 1 -
CHEMBL356362 ISOPENTENYLPYROPHOSPHATE 1 -
CHEMBL4116247 1 -
CHEMBL4060737 1 -
CHEMBL4521062 1 -
CHEMBL4061531 1 -
CHEMBL4088948 1 -
CHEMBL4531623 1 -
CHEMBL450079 SODIUM PYROPHOSPHATE -1.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4302450 IC50 = 7870.0 nM 5.10
CHEMBL145233 IC50 = 23000.0 nM 4.64
CHEMBL343480 DIMETHYLALLYL DIPHOSPHATE IC50 = 270000.0 nM -
CHEMBL356362 ISOPENTENYLPYROPHOSPHATE IC50 = 513000.0 nM -
CHEMBL4116247 IC50 = 103000.0 nM -
CHEMBL4060737 IC50 = 2560000.0 nM -
CHEMBL4521062 IC50 = 1040000.0 nM -
CHEMBL4061531 IC50 - -
CHEMBL4088948 IC50 - -
CHEMBL4531623 IC50 - -
CHEMBL450079 SODIUM PYROPHOSPHATE IC50 = 1140000.0 nM -