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Assay Detail

CHEMBL4368093

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human DPP4 expressed in baculovirus infected Sf9 insect cells using Gly-Pro-AMC as substrate preincubated for 15 mins followed by substrate addition measured at 60 secs interval for 20 mins by fluorometic method
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dipeptidyl peptidase 4 (CHEMBL284)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rapid generation of novel benzoic acid-based xanthine derivatives as highly potent, selective and long acting DPP-4 inhibitors: Scaffold-hopping and prodrug study.
Li Q, Meng L, Zhou S, Deng X, Wang N, Ji Y, Peng Y, Xing J, Yao G.
Eur J Med Chem (2019) 180 : 509 -523
PubMed 31336309 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 8.47 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4448186 1 10.00
CHEMBL4465482 1 9.74
CHEMBL4461587 1 9.66
CHEMBL4588692 1 9.36
CHEMBL4536341 1 9.14
CHEMBL4544024 1 8.98
CHEMBL4452518 1 8.70
CHEMBL4446618 1 8.66
CHEMBL4528994 1 8.63
CHEMBL4464069 1 8.61
CHEMBL4435383 1 8.54
CHEMBL4458949 1 8.41
CHEMBL376359 ALOGLIPTIN 4.0 1 8.35
CHEMBL4590999 1 8.30
CHEMBL4458152 1 8.25
CHEMBL4458832 1 8.22
CHEMBL4441220 1 7.99
CHEMBL4567320 1 7.69
CHEMBL4465374 1 7.66
CHEMBL4465448 1 7.29
CHEMBL4468590 1 7.13
CHEMBL4528149 1 7.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4448186 IC50 = 0.1 nM 10.00
CHEMBL4465482 IC50 = 0.18 nM 9.74
CHEMBL4461587 IC50 = 0.22 nM 9.66
CHEMBL4588692 IC50 = 0.44 nM 9.36
CHEMBL4536341 IC50 = 0.72 nM 9.14
CHEMBL4544024 IC50 = 1.05 nM 8.98
CHEMBL4452518 IC50 = 1.98 nM 8.70
CHEMBL4446618 IC50 = 2.19 nM 8.66
CHEMBL4528994 IC50 = 2.35 nM 8.63
CHEMBL4464069 IC50 = 2.43 nM 8.61
CHEMBL4435383 IC50 = 2.91 nM 8.54
CHEMBL4458949 IC50 = 3.9 nM 8.41
CHEMBL376359 ALOGLIPTIN IC50 = 4.46 nM 8.35
CHEMBL4590999 IC50 = 5.06 nM 8.30
CHEMBL4458152 IC50 = 5.61 nM 8.25
CHEMBL4458832 IC50 = 5.99 nM 8.22
CHEMBL4441220 IC50 = 10.21 nM 7.99
CHEMBL4567320 IC50 = 20.48 nM 7.69
CHEMBL4465374 IC50 = 22.09 nM 7.66
CHEMBL4465448 IC50 = 50.9 nM 7.29
CHEMBL4468590 IC50 = 74.43 nM 7.13
CHEMBL4528149 IC50 = 85.48 nM 7.07