Assay Detail
Binding
CHEMBL4368174
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human 5-LOX expressed in Escherichia coli BL21 using arachidonic acid as substrate preincubated for 15 mins followed by substrate addition by HPLC analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL215) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Protective effect of piceatannol and bioactive stilbene derivatives against hypoxia-induced toxicity in H9c2 cardiomyocytes and structural elucidation as 5-LOX inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 5.78 | 6.50 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL332610 | — | — | 1 | 6.50 |
| CHEMBL119576 | — | — | 1 | 6.11 |
| CHEMBL69863 | PICEATANNOL | — | 1 | 5.96 |
| CHEMBL296411 | TRISMETHOXYRESVERATROL | — | 1 | 5.44 |
| CHEMBL83527 | PTEROSTILBENE | 2.0 | 1 | 5.41 |
| CHEMBL419378 | — | — | 1 | 5.26 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL332610 | — | IC50 | = | 320.0 | nM | 6.50 |
| CHEMBL119576 | — | IC50 | = | 780.0 | nM | 6.11 |
| CHEMBL69863 | PICEATANNOL | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL296411 | TRISMETHOXYRESVERATROL | IC50 | = | 3620.0 | nM | 5.44 |
| CHEMBL83527 | PTEROSTILBENE | IC50 | = | 3910.0 | nM | 5.41 |
| CHEMBL419378 | — | IC50 | = | 5490.0 | nM | 5.26 |