Assay Detail
Binding
CHEMBL4368757
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of TGF beta1/Smad3 signaling in mouse NIH/3T3 cells harboring CAGA promoter incubated for 18 hrs in presence of TGF-beta1 by luciferase reporter gene assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Confidence | 5 — Multiple protein complex / RNA |
| Curated By | Autocuration |
Target
Transforming growth factor beta-1 proprotein/Mothers against decapentaplegic homolog 3 (CHEMBL4296078) ↗| Type | PROTEIN-PROTEIN INTERACTION |
| Organism | Mus musculus |
Publication
In vitro screening for compounds from Hypericum longistylum with anti-pulmonary fibrosis activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 6.21 | 7.15 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL401570 | — | — | 1 | 7.15 |
| CHEMBL4475683 | — | — | 1 | 6.42 |
| CHEMBL1256391 | PIRFENIDONE | 4.0 | 1 | 5.84 |
| CHEMBL3315225 | — | — | 1 | 5.43 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL401570 | — | IC50 | = | 70.51 | nM | 7.15 |
| CHEMBL4475683 | — | IC50 | = | 380.0 | nM | 6.42 |
| CHEMBL1256391 | PIRFENIDONE | IC50 | = | 1430.0 | nM | 5.84 |
| CHEMBL3315225 | — | IC50 | = | 3670.0 | nM | 5.43 |