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Assay Detail

CHEMBL4369260

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition and measured after 10 mins by Ellman's method
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Electrophorus electricus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL4078)
Type SINGLE PROTEIN
Organism Electrophorus electricus

Publication

Selected aryl thiosemicarbazones as a new class of multi-targeted monoamine oxidase inhibitors.
Mathew B, Baek SC, Grace Thomas Parambi D, Pil Lee J, Joy M, Annie Rilda PR, Randev RV, Nithyamol P, Vijayan V, Inasu ST, Mathew GE, Lohidakshan KK, Kumar Krishnan G, Kim H.
Medchemcomm (2018) 9 : 1871 -1881
PubMed 30568755 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 4.74 6.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL95 TACRINE 4.0 1 6.64
CHEMBL193804 1 4.81
CHEMBL1818874 1 4.58
CHEMBL1096480 1 4.57
CHEMBL1910220 1 4.51
CHEMBL393467 1 4.51
CHEMBL241902 1 4.45
CHEMBL398206 1 4.44
CHEMBL2369206 1 4.44
CHEMBL1818875 1 4.42
CHEMBL243185 PHENYLTHIOSEMICARBAZONE 1 -
CHEMBL242113 1 -
CHEMBL1096479 1 -
CHEMBL391982 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL95 TACRINE IC50 = 230.0 nM 6.64
CHEMBL193804 IC50 = 15610.0 nM 4.81
CHEMBL1818874 IC50 = 26610.0 nM 4.58
CHEMBL1096480 IC50 = 26720.0 nM 4.57
CHEMBL1910220 IC50 = 31120.0 nM 4.51
CHEMBL393467 IC50 = 30840.0 nM 4.51
CHEMBL241902 IC50 = 35350.0 nM 4.45
CHEMBL398206 IC50 = 36140.0 nM 4.44
CHEMBL2369206 IC50 = 36230.0 nM 4.44
CHEMBL1818875 IC50 = 37930.0 nM 4.42
CHEMBL243185 PHENYLTHIOSEMICARBAZONE IC50 > 40000.0 nM -
CHEMBL242113 IC50 > 40000.0 nM -
CHEMBL1096479 IC50 > 40000.0 nM -
CHEMBL391982 IC50 > 40000.0 nM -