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Assay Detail

CHEMBL4371734

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NO711 binding to mouse GAT1 expressed in HEK293 cell membranes assessed as residual binding preincubated for 4 hrs followed by NO711 addition and measured after 40 mins by LC-ESI-MS/MS analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent GABA transporter 1 (CHEMBL5445)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Novel Allosteric Ligands of γ-Aminobutyric Acid Transporter 1 (GAT1) by MS Based Screening of Pseudostatic Hydrazone Libraries.
Hauke TJ, Wein T, Höfner G, Wanner KT.
J Med Chem (2018) 61 : 10310 -10332
PubMed 30376325 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 6.19 7.56

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1027 TIAGABINE 4.0 1 7.56
CHEMBL4525620 1 6.67
CHEMBL4537310 1 6.61
CHEMBL4560424 1 6.35
CHEMBL4563656 1 6.33
CHEMBL4526583 1 6.32
CHEMBL4436249 1 6.21
CHEMBL4539491 1 6.19
CHEMBL4448463 1 6.15
CHEMBL4468482 1 6.03
CHEMBL4456820 1 6.02
CHEMBL4573932 1 5.91
CHEMBL4539900 1 5.90
CHEMBL4457922 1 5.84
CHEMBL4450658 1 5.83
CHEMBL4570110 1 5.73
CHEMBL4455881 1 5.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1027 TIAGABINE Ki = 27.54 nM 7.56
CHEMBL4525620 Ki = 213.8 nM 6.67
CHEMBL4537310 Ki = 245.47 nM 6.61
CHEMBL4560424 Ki = 446.68 nM 6.35
CHEMBL4563656 Ki = 467.74 nM 6.33
CHEMBL4526583 Ki = 478.63 nM 6.32
CHEMBL4436249 Ki = 616.6 nM 6.21
CHEMBL4539491 Ki = 645.65 nM 6.19
CHEMBL4448463 Ki = 707.95 nM 6.15
CHEMBL4468482 Ki = 933.25 nM 6.03
CHEMBL4456820 Ki = 954.99 nM 6.02
CHEMBL4573932 Ki = 1230.27 nM 5.91
CHEMBL4539900 Ki = 1258.93 nM 5.90
CHEMBL4457922 Ki = 1445.44 nM 5.84
CHEMBL4450658 Ki = 1479.11 nM 5.83
CHEMBL4570110 Ki = 1862.09 nM 5.73
CHEMBL4455881 Ki = 2290.87 nM 5.64