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Assay Detail

CHEMBL4372250

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of thermolysin activated recombinant human C-terminal 10-His tagged KLK7 (E23 to H252 residues) using MOCAc-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by fluorescence analysis
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kallikrein-7 (CHEMBL2443)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and structure-activity relationship of imidazolinylindole derivatives as kallikrein 7 inhibitors.
Murafuji H, Muto T, Goto M, Imajo S, Sugawara H, Oyama Y, Minamitsuji Y, Miyazaki S, Murai K, Fujioka H.
Bioorg Med Chem Lett (2019) 29 : 334 -338
PubMed 30522951 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 5.16 6.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4554224 1 6.41
CHEMBL4517387 1 6.00
CHEMBL4575145 1 5.92
CHEMBL4567825 1 5.72
CHEMBL4467792 1 5.39
CHEMBL4453721 1 5.33
CHEMBL4572471 1 5.24
CHEMBL4557205 1 5.17
CHEMBL4442779 1 5.03
CHEMBL4566954 1 5.00
CHEMBL4438568 1 4.96
CHEMBL4528949 1 4.85
CHEMBL4520107 1 4.77
CHEMBL4567713 1 4.66
CHEMBL4571184 1 4.62
CHEMBL4444444 1 4.52
CHEMBL4540373 1 4.16
CHEMBL4549943 1 -
CHEMBL4577042 1 -
CHEMBL4535920 1 -
CHEMBL4438651 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4554224 IC50 = 390.0 nM 6.41
CHEMBL4517387 IC50 = 1000.0 nM 6.00
CHEMBL4575145 IC50 = 1200.0 nM 5.92
CHEMBL4567825 IC50 = 1900.0 nM 5.72
CHEMBL4467792 IC50 = 4100.0 nM 5.39
CHEMBL4453721 IC50 = 4700.0 nM 5.33
CHEMBL4572471 IC50 = 5700.0 nM 5.24
CHEMBL4557205 IC50 = 6700.0 nM 5.17
CHEMBL4442779 IC50 = 9400.0 nM 5.03
CHEMBL4566954 IC50 = 10000.0 nM 5.00
CHEMBL4438568 IC50 = 11000.0 nM 4.96
CHEMBL4528949 IC50 = 14000.0 nM 4.85
CHEMBL4520107 IC50 = 17000.0 nM 4.77
CHEMBL4567713 IC50 = 22000.0 nM 4.66
CHEMBL4571184 IC50 = 24000.0 nM 4.62
CHEMBL4444444 IC50 = 30000.0 nM 4.52
CHEMBL4540373 IC50 = 70000.0 nM 4.16
CHEMBL4549943 IC50 > 100000.0 nM -
CHEMBL4577042 IC50 > 30000.0 nM -
CHEMBL4535920 IC50 > 100000.0 nM -
CHEMBL4438651 IC50 > 30000.0 nM -