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Assay Detail

CHEMBL4372252

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of enterokinase activated recombinant mouse C-terminal His6-tagged KLK7 (Gln22 to Arg249 residues) using MOCAc-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by fluorescence analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kallikrein-7 (CHEMBL4295910)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery and structure-activity relationship of imidazolinylindole derivatives as kallikrein 7 inhibitors.
Murafuji H, Muto T, Goto M, Imajo S, Sugawara H, Oyama Y, Minamitsuji Y, Miyazaki S, Murai K, Fujioka H.
Bioorg Med Chem Lett (2019) 29 : 334 -338
PubMed 30522951 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.30 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4554224 1 6.40
CHEMBL4517387 1 5.89
CHEMBL4575145 1 5.82
CHEMBL4467792 1 5.19
CHEMBL4444444 1 5.00
CHEMBL4528949 1 5.00
CHEMBL4557205 1 4.96
CHEMBL4520107 1 4.82
CHEMBL4438568 1 4.66
CHEMBL4567825 1 -
CHEMBL4442779 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4554224 IC50 = 400.0 nM 6.40
CHEMBL4517387 IC50 = 1300.0 nM 5.89
CHEMBL4575145 IC50 = 1500.0 nM 5.82
CHEMBL4467792 IC50 = 6400.0 nM 5.19
CHEMBL4444444 IC50 = 10000.0 nM 5.00
CHEMBL4528949 IC50 = 10000.0 nM 5.00
CHEMBL4557205 IC50 = 11000.0 nM 4.96
CHEMBL4520107 IC50 = 15000.0 nM 4.82
CHEMBL4438568 IC50 = 22000.0 nM 4.66
CHEMBL4567825 IC50 > 100000.0 nM -
CHEMBL4442779 IC50 > 30000.0 nM -