Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4375085

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of N-terminal fluorescein labelled Puma-BH3 peptide from human N-terminal thrombin cleavage site-fused/His6-tagged Bcl-2 expressed in Escherichia coliBL21(DE3) pLysS cells by fluorescence polarization assay
23
Total Activities
22
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apoptosis regulator Bcl-2 (CHEMBL4860)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Guided Discovery of a Selective Mcl-1 Inhibitor with Cellular Activity.
Szlávik Z, Ondi L, Csékei M, Paczal A, Szabó ZB, Radics G, Murray J, Davidson J, Chen I, Davis B, Hubbard RE, Pedder C, Dokurno P, Surgenor A, Smith J, Robertson A, LeToumelin-Braizat G, Cauquil N, Zarka M, Demarles D, Perron-Sierra F, Claperon A, Colland F, Geneste O, Kotschy A.
J Med Chem (2019) 62 : 6913 -6924
PubMed 31339316 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 22 4.78 5.89
IC50 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4521569 1 5.89
CHEMBL4472592 1 5.39
CHEMBL4521112 1 5.30
CHEMBL4464302 1 5.27
CHEMBL4467044 1 5.21
CHEMBL4455822 1 5.09
CHEMBL4460382 1 4.89
CHEMBL4524567 1 4.68
CHEMBL4514111 1 4.62
CHEMBL4450255 1 4.62
CHEMBL4450405 1 4.55
CHEMBL4515659 1 4.54
CHEMBL4452148 1 4.44
CHEMBL4443675 1 4.31
CHEMBL4442085 1 4.29
CHEMBL4572199 2 4.13
CHEMBL4573285 1 4.01
CHEMBL4458787 1 -
CHEMBL4455139 1 -
CHEMBL4471326 1 -
CHEMBL4440978 1 -
CHEMBL4460632 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4521569 Ki = 1300.0 nM 5.89
CHEMBL4472592 Ki = 4100.0 nM 5.39
CHEMBL4521112 Ki = 5000.0 nM 5.30
CHEMBL4464302 Ki = 5400.0 nM 5.27
CHEMBL4467044 Ki = 6100.0 nM 5.21
CHEMBL4455822 Ki = 8100.0 nM 5.09
CHEMBL4460382 Ki = 13000.0 nM 4.89
CHEMBL4524567 Ki = 21000.0 nM 4.68
CHEMBL4514111 Ki = 24000.0 nM 4.62
CHEMBL4450255 Ki = 24000.0 nM 4.62
CHEMBL4450405 Ki = 28000.0 nM 4.55
CHEMBL4515659 Ki = 29000.0 nM 4.54
CHEMBL4452148 Ki = 36000.0 nM 4.44
CHEMBL4443675 Ki = 49000.0 nM 4.31
CHEMBL4442085 Ki = 51000.0 nM 4.29
CHEMBL4572199 Ki = 74000.0 nM 4.13
CHEMBL4573285 Ki = 98000.0 nM 4.01
CHEMBL4572199 IC50 = 164000.0 nM -
CHEMBL4458787 Ki = 418000.0 nM -
CHEMBL4440978 Ki = 359000.0 nM -
CHEMBL4455139 Ki = 470000.0 nM -
CHEMBL4471326 Ki = 269000.0 nM -
CHEMBL4460632 Ki = 437000.0 nM -