Assay Detail
Binding
CHEMBL4376265
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to full length Mycobacterium tuberculosis tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mycobacterium tuberculosis |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
tRNA (guanine-N(1)-)-methyltransferase (CHEMBL4523933) ↗| Type | SINGLE PROTEIN |
| Organism | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Publication
Thienopyrimidinone Derivatives That Inhibit Bacterial tRNA (Guanine37-<i>N</i><sup>1</sup>)-Methyltransferase (TrmD) by Restructuring the Active Site with a Tyrosine-Flipping Mechanism.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 3 | 5.57 | 6.72 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4517402 | — | — | 1 | 6.72 |
| CHEMBL418052 | S-ADENOSYLHOMOCYSTEINE | — | 1 | 5.00 |
| CHEMBL2431997 | — | — | 1 | 4.98 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4517402 | — | Kd | = | 190.0 | nM | 6.72 |
| CHEMBL418052 | S-ADENOSYLHOMOCYSTEINE | Kd | = | 10100.0 | nM | 5.00 |
| CHEMBL2431997 | — | Kd | = | 10400.0 | nM | 4.98 |