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Assay Detail

CHEMBL4380948

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Binding
Inhibition of MNK1/MNK2 in human HeLa cells overexpressing eIF4E assessed as reduction in eIF4E phosphorylation at Ser209 residue after 2 hrs by Alphascreen SureFly assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Publication

Stepwise Evolution of Fragment Hits against MAPK Interacting Kinases 1 and 2.
Kwiatkowski J, Liu B, Pang S, Ahmad NHB, Wang G, Poulsen A, Yang H, Poh YR, Tee DHY, Ong E, Retna P, Dinie N, Kwek P, Wee JLK, Manoharan V, Low CB, Seah PG, Pendharkar V, Sangthongpitag K, Joy J, Baburajendran N, Jansson AE, Nacro K, Hill J, Keller TH, Hung AW.
J Med Chem (2020) 63 : 621 -637
PubMed 31910010 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.17 6.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4442209 1 6.80
CHEMBL4587832 1 6.70
CHEMBL4569209 1 6.50
CHEMBL4462099 1 6.43
CHEMBL4443472 1 6.41
CHEMBL4587472 1 6.24
CHEMBL4457274 1 6.12
CHEMBL4450463 1 6.07
CHEMBL4588193 1 6.03
CHEMBL4441602 1 5.77
CHEMBL4586717 1 5.72
CHEMBL4465294 1 5.70
CHEMBL4440223 1 5.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4442209 IC50 = 160.0 nM 6.80
CHEMBL4587832 IC50 = 200.0 nM 6.70
CHEMBL4569209 IC50 = 320.0 nM 6.50
CHEMBL4462099 IC50 = 370.0 nM 6.43
CHEMBL4443472 IC50 = 390.0 nM 6.41
CHEMBL4587472 IC50 = 570.0 nM 6.24
CHEMBL4457274 IC50 = 750.0 nM 6.12
CHEMBL4450463 IC50 = 860.0 nM 6.07
CHEMBL4588193 IC50 = 940.0 nM 6.03
CHEMBL4441602 IC50 = 1700.0 nM 5.77
CHEMBL4586717 IC50 = 1900.0 nM 5.72
CHEMBL4465294 IC50 = 2000.0 nM 5.70
CHEMBL4440223 IC50 = 2200.0 nM 5.66