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Assay Detail

CHEMBL4384327

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Hedgehog signaling pathway in mouse C3H10T1/2 cells assessed as reduction in oxysterol-induced Gli1 mRNA expression after 24 hrs by q-PCR analysis
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis and evaluation of third generation vitamin D3 analogues as inhibitors of Hedgehog signaling.
Maschinot CA, Chau LQ, Wechsler-Reya RJ, Hadden MK.
Eur J Med Chem (2019) 162 : 495 -506
PubMed 30471551 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.22 9.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL197589 LG-190178 1 9.60
CHEMBL846 CALCITRIOL 4.0 1 8.51
CHEMBL1040 CALCIFEDIOL ANHYDROUS 4.0 1 6.40
CHEMBL4449816 1 6.40
CHEMBL3408015 1 6.40
CHEMBL4542767 1 6.22
CHEMBL4580825 1 6.05
CHEMBL4524451 1 5.96
CHEMBL4459512 1 5.89
CHEMBL4522782 1 5.80
CHEMBL4439766 1 5.80
CHEMBL4438180 1 5.77
CHEMBL4459996 1 5.64
CHEMBL4582806 1 5.51
CHEMBL4443970 1 5.42
CHEMBL473417 VISMODEGIB 4.0 1 4.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL197589 LG-190178 IC50 = 0.25 nM 9.60
CHEMBL846 CALCITRIOL IC50 = 3.1 nM 8.51
CHEMBL1040 CALCIFEDIOL ANHYDROUS IC50 = 400.0 nM 6.40
CHEMBL4449816 IC50 = 400.0 nM 6.40
CHEMBL3408015 IC50 = 400.0 nM 6.40
CHEMBL4542767 IC50 = 600.0 nM 6.22
CHEMBL4580825 IC50 = 890.0 nM 6.05
CHEMBL4524451 IC50 = 1100.0 nM 5.96
CHEMBL4459512 IC50 = 1300.0 nM 5.89
CHEMBL4522782 IC50 = 1600.0 nM 5.80
CHEMBL4439766 IC50 = 1600.0 nM 5.80
CHEMBL4438180 IC50 = 1700.0 nM 5.77
CHEMBL4459996 IC50 = 2300.0 nM 5.64
CHEMBL4582806 IC50 = 3100.0 nM 5.51
CHEMBL4443970 IC50 = 3800.0 nM 5.42
CHEMBL473417 VISMODEGIB IC50 = 82000.0 nM 4.09